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sequence-specific stem-loop rt primers taqman microrna reverse transcription kit  (Thermo Fisher)


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    Thermo Fisher sequence-specific stem-loop rt primers taqman microrna reverse transcription kit
    Sequence Specific Stem Loop Rt Primers Taqman Microrna Reverse Transcription Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sequence-specific+primers+kit/sequence+specific+stem+loop+rt+primers+taqman+microrna+reverse+transcription+kit/pm40412229-94-1-12
    Average 90 stars, based on 1 article reviews
    sequence-specific stem-loop rt primers taqman microrna reverse transcription kit - by Bioz Stars, 2026-10
    90/100 stars

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    other:

    Article Title: Unexpected rise in the circulation of complex HBV variants enriched of HBsAg vaccine-escape mutations in HBV genotype-D: potential impact on HBsAg detection/quantification and vaccination strategies.
    Article Snippet: PCR-products were purified and sequenced by using different sequence-specific primers and a BigDye-Terminator v.3.1 cycle sequencing kit (AppliedBiosystems, Foster City, USA) on the ABI Prism 3130XL (Applied-Biosystems, Foster City, USA).

    Article Title: Unexpected rise in the circulation of complex HBV variants enriched of HBsAg vaccine-escape mutations in HBV genotype-D: potential impact on HBsAg detection/quantification and vaccination strategies
    Article Snippet: PCR-products were purified and sequenced by using different sequence-specific primers and a BigDye-Terminator v.3.1 cycle sequencing kit (Applied-Biosystems, Foster City, USA) on the ABI Prism 3130XL (Applied-Biosystems, Foster City, USA).

    Article Title: Molecular and Genetic Characterization of Hepatitis B Virus (HBV) among Saudi Chronically HBV-Infected Individuals
    Article Snippet: PCR products were sequenced by using eight different overlapping sequence-specific primers with a Big Dye terminator v. 3.1 cycle sequencing kit (Applied Biosystems, Foster City, CA, USA) with the Sanger sequencing method, and an automated sequencer (ABI-3100), as reported elsewhere [ , ].

    Article Title: Molecular and Genetic Characterization of Hepatitis B Virus (HBV) among Saudi Chronically HBV-Infected Individuals.
    Article Snippet: PCR products were sequenced by using eight different overlapping sequence-specific primers with a Big Dye terminator v. 3.1 cycle sequencing kit (Applied Biosystems, Foster City, CA, USA) with the Sanger sequencing method, and an automated sequencer (ABI3100), as reported elsewhere [18,19].

    Article Title: A new genetic cause of spastic ataxia: the p.Glu415Lys variant in TUBA4A.
    Article Snippet: Direct Sanger sequencing using specific primers was performed with a BigDye v3.1 sequencing kit (Applied Biosystems, Waltham, MA, USA) on a 3500xl Genetic Analyzer (Applied Biosystems).

    SYBR Green Assay:

    Article Title: The Nephroprotective Effect of MS-275 on Lipopolysaccharide (LPS)-Induced Acute Kidney Injury by Inhibiting Reactive Oxygen Species (ROS)-Oxidative Stress and Endoplasmic Reticulum Stress
    Article Snippet: .. The q-PCR was performed with cDNA using the SYBR Green kit, gene-specific primers and a 7500 Sequence Detector System (Applied Biosystems, Foster City, CA, USA). .. The primers ( ) were developed with Primer Express software (Applied Biosystems).

    Sequencing:

    Article Title: The Nephroprotective Effect of MS-275 on Lipopolysaccharide (LPS)-Induced Acute Kidney Injury by Inhibiting Reactive Oxygen Species (ROS)-Oxidative Stress and Endoplasmic Reticulum Stress
    Article Snippet: .. The q-PCR was performed with cDNA using the SYBR Green kit, gene-specific primers and a 7500 Sequence Detector System (Applied Biosystems, Foster City, CA, USA). .. The primers ( ) were developed with Primer Express software (Applied Biosystems).

    Article Title: Functional characterization of two melanocortin (MC) receptors in lamprey showing orthology to the MC1 and MC4 receptor subtypes
    Article Snippet: Touch-down PCR was performed with Taq polymerase (Fermentas, Lithuania) under the following conditions: initial denaturation for 5 min at 95 C, followed by 21 cycle of 30 s at 94 C, 45 s at 52-42 C (lowered by 0.5 C per cycle) and 90 s at 72 C. This was followed by 25 cycles of 30 s at 94 C, 40 s at 50 C and 1 min at 72 C, with a final extension of 5 min at 72 C. The PCR products were separated on a 1.5% agarose gel, and the products of desired length were cut out from the gel, purified using the Gel Extraction Kit (Qiagen, Sweden) and cloned into the plasmid vector pCRII from TOPO cloning kit and transformed into DH5α cells (Invitrogen, Sweden) according to the manufacturers' instructions. .. The plasmid inserts were sequenced with vector-specific primers using ABI PRISM Big Dye Terminator Cycle Sequencing Ready Reaction Kit v2.0 and analyzed by an automated ABI PRISM 310 or ABI PRISM 3100 fluorescent-dye sequencer (Applied Biosystems, Sweden). .. Sequences were analysed with Seqman software from DNAstar package (Lasergene, USA).

    Article Title: Brown Seaweed Fucoidan Inhibits Cancer Progression by Dual Regulation of mir-29c/ADAM12 and miR-17-5p/PTEN Axes in Human Breast Cancer Cells
    Article Snippet: After cells were treated with fucoidan for 48 h, total RNA was isolated using Trizol (Invitrogen, Grand Island, NY, USA) according to the manufacturer's instructions. .. To analyze the expression of mature miR-29c and miR-17-5p, complementary cDNA was synthesized using sequence-specific primers and the TaqMan MicroRNA Reverse Transcription Kit (Applied Biosystems, Foster City, CA, USA). .. For cDNA amplification, quantitative reverse-transcription polymerase chain reaction (qRT-PCR) was performed on the ABI 7500 real-time system (Applied Biosystems) by using sequence-specific primers from the TaqMan MicroRNA Assays Kit.

    Plasmid Preparation:

    Article Title: Functional characterization of two melanocortin (MC) receptors in lamprey showing orthology to the MC1 and MC4 receptor subtypes
    Article Snippet: Touch-down PCR was performed with Taq polymerase (Fermentas, Lithuania) under the following conditions: initial denaturation for 5 min at 95 C, followed by 21 cycle of 30 s at 94 C, 45 s at 52-42 C (lowered by 0.5 C per cycle) and 90 s at 72 C. This was followed by 25 cycles of 30 s at 94 C, 40 s at 50 C and 1 min at 72 C, with a final extension of 5 min at 72 C. The PCR products were separated on a 1.5% agarose gel, and the products of desired length were cut out from the gel, purified using the Gel Extraction Kit (Qiagen, Sweden) and cloned into the plasmid vector pCRII from TOPO cloning kit and transformed into DH5α cells (Invitrogen, Sweden) according to the manufacturers' instructions. .. The plasmid inserts were sequenced with vector-specific primers using ABI PRISM Big Dye Terminator Cycle Sequencing Ready Reaction Kit v2.0 and analyzed by an automated ABI PRISM 310 or ABI PRISM 3100 fluorescent-dye sequencer (Applied Biosystems, Sweden). .. Sequences were analysed with Seqman software from DNAstar package (Lasergene, USA).

    Expressing:

    Article Title: Brown Seaweed Fucoidan Inhibits Cancer Progression by Dual Regulation of mir-29c/ADAM12 and miR-17-5p/PTEN Axes in Human Breast Cancer Cells
    Article Snippet: After cells were treated with fucoidan for 48 h, total RNA was isolated using Trizol (Invitrogen, Grand Island, NY, USA) according to the manufacturer's instructions. .. To analyze the expression of mature miR-29c and miR-17-5p, complementary cDNA was synthesized using sequence-specific primers and the TaqMan MicroRNA Reverse Transcription Kit (Applied Biosystems, Foster City, CA, USA). .. For cDNA amplification, quantitative reverse-transcription polymerase chain reaction (qRT-PCR) was performed on the ABI 7500 real-time system (Applied Biosystems) by using sequence-specific primers from the TaqMan MicroRNA Assays Kit.

    Synthesized:

    Article Title: Brown Seaweed Fucoidan Inhibits Cancer Progression by Dual Regulation of mir-29c/ADAM12 and miR-17-5p/PTEN Axes in Human Breast Cancer Cells
    Article Snippet: After cells were treated with fucoidan for 48 h, total RNA was isolated using Trizol (Invitrogen, Grand Island, NY, USA) according to the manufacturer's instructions. .. To analyze the expression of mature miR-29c and miR-17-5p, complementary cDNA was synthesized using sequence-specific primers and the TaqMan MicroRNA Reverse Transcription Kit (Applied Biosystems, Foster City, CA, USA). .. For cDNA amplification, quantitative reverse-transcription polymerase chain reaction (qRT-PCR) was performed on the ABI 7500 real-time system (Applied Biosystems) by using sequence-specific primers from the TaqMan MicroRNA Assays Kit.

    Reverse Transcription:

    Article Title: Brown Seaweed Fucoidan Inhibits Cancer Progression by Dual Regulation of mir-29c/ADAM12 and miR-17-5p/PTEN Axes in Human Breast Cancer Cells
    Article Snippet: After cells were treated with fucoidan for 48 h, total RNA was isolated using Trizol (Invitrogen, Grand Island, NY, USA) according to the manufacturer's instructions. .. To analyze the expression of mature miR-29c and miR-17-5p, complementary cDNA was synthesized using sequence-specific primers and the TaqMan MicroRNA Reverse Transcription Kit (Applied Biosystems, Foster City, CA, USA). .. For cDNA amplification, quantitative reverse-transcription polymerase chain reaction (qRT-PCR) was performed on the ABI 7500 real-time system (Applied Biosystems) by using sequence-specific primers from the TaqMan MicroRNA Assays Kit.



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